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CABRI:NCCB_PLASMID |
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Collection_number |
NCCB 3028
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Name |
pMC1871
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Other_culture_collection_numbers |
PC-V3028
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Type |
plasmid
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Class |
Recombinant
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Literature |
Casadaban, M.J. et al., Methods Enzymol. (1983) 100:293-308 [PMID: 6312261
] |
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History_of_deposit |
-
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Restricted_distribution |
No
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Host_for_distribution |
Escherichia coli K12 AB1157
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Medium |
Medium 171
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Selectable_phenotype |
Tetracycline resistance (Tc)
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Replicon |
pMB1
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Transfer_ability |
Non-transferable
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Host_range |
Escherichia coli vector
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Properties_and_applications |
general cloning vector
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Molecular_weight |
7.4 kb
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Cloned_gene |
lacZ-
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Further_information |
the non-unique restriction sites as well as HindIII and SmaI can be used to
excise the lacZ gene (lacking the control region and the first 8 non-essential codons); can be
used as a source for the lacZ gene, e.g. to study control of expression of other genes, or to form
protein fusions
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Restriction_sites |
():BamHI,EcoRI,PstI,SalI; (U):AvaI,HindIII,PvuII,SmaI
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Price_code |
1
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